Figure 3.

EBV infection amplifies EGF-stimulated Ca²⁺ signaling via SOCE.

Notes: (A) EGF-evoked Ca²⁺ transient responses were measured in the fura-2-loaded cells. The dynamic cytosolic Ca²⁺ level [Ca²⁺]_cyt was measured as the fluorescence ratio (F340/F380) of fura-2. Each trace represents the average data from at least 20 individual cells. The intensities of Ca²⁺ responses were quantitatively evaluated by calculating the average peak from the baseline in each right panel. (B) EGF-induced Ca²⁺ release from intracellular Ca²⁺ stores (ER) and the following Ca²⁺ influx via membrane channels were measured in the absence and presence of extracellular Ca²⁺, successively. The data are representative of three independent experiments and are presented as the mean ± SEM (*P<0.05, Student’s t-test).

Abbreviations: EBV, Epstein–Barr virus; EGF, epidermal growth factor; ER, endoplasmic reticulum.