Figure 3.
Congenital lung lesion cysts are lined by differentiated airway epithelium. (A–L) The epithelial lining of all types of congenital lung lesions was noted to express mature secretory (SCGB3A2), ciliated (TUBB4A), and basal cell (TP63 [green]/KRT5 [red]) markers. (M) Significantly increased expression of numerous genes in the airway epithelium was observed by gene expression arrays. Comparison testing was performed with the software package limma, based on a false discovery rate less than 5% accounting for multiple comparisons. Comparisons were significant using these criteria, except where noted. CPAM = congenital pulmonary airway malformation; KRT5 = keratin, type II cytoskeletal 5; NS = not significant; RMA = robust multiarray average; SCGB3A2 = secretoglobin 3A2; TP63 = tumor protein 63; TUBB4A = tubulin, β4A class. N = 12 patients. Scale bars, 50 μm.