Figure 3. Maitake D-Fraction decreases the activity of MMP-2 and MMP-9 secreted by TNBC MDA-MB-231 cells.

(A) Representative image of gelatinolytic bands obtained with the different CM and visualized using a transilluminator (left) and its parallel coomassie gel (right). (B) Activity of the MMPs after D-Fraction treatment, plotted as a percentage of the control. Gelatinolytic bands were measured with Fiji (Fiji Is Just ImageJ) and normalized to total protein concentration. Values are the mean ± SEM of three independent experiments. Two-way ANOVA and Bonferroni post tests. ^*p < 0.05, ^***p < 0.001.