Figure 1. Schematic representation of the experimental approach used to identify PTPRJ-interacting proteins.

(A) A549 lung cancer cells were transduced with a recombinant adenovirus carrying a cDNA coding for PTPRJ fused to a His6-tag. (B) transduced A549 cells were lysed and enriched membrane proteins were extracted. (C) PTPRJ-His6 and its candidate interacting-proteins were isolated through poly(His)-avid magnetic beads; (D) beads-bound proteins were eluted and (E) analyzed for peptides identification by mass spectrometry.