Figure 2. Analysis of cell death of CD24⁺/CD44^low cells and CD24^−/low/CD44⁺ cells after 10 Gy irradiation.

(A) Time course of cell death of 10 Gy-irradiated CD24⁺/CD44^low cells and CD24^−/low/CD44⁺ cells. Results correspond to the mean ± SD of 2 to 4 independent experiments. (B) Kinetics of cleavage of caspase 7 at different times after 10 Gy irradiation. The Western blot shown is representative of 3 independent experiments. (C) Flow cytometry characterization of cleaved caspase 3 before and 4 days after irradiation. Dotted line corresponds to isotypic control. Similar results were obtained in 3 different experiments. (D) Clonogenic cell survival curves for CD24⁺/CD44^low cells and CD24^−/low/CD44⁺ cells exposed to 2 and 4 Gy irradiation. Error bars represent standard error from the mean for 3 separate experiments.