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. 2018 Apr 24;9(31):21641–21654. doi: 10.18632/oncotarget.24956

Table 4. Sequences of primers and TaqMan fluorogenic probes.

Gene Sequence (5′-3′) Product size (bp)
r18s
Forward Primer cggctaccacatccaaggaa 197
Reverse Primer cgctattggagctggaatta
Probe (HEX) – tgctggcaccagacttgccctc– (BHQ-1)
CK19
Forward Primer tcgacaacgcccgtctg 75
Reverse Primer ccacgctcatgcgcag
Probe (6FAM) – cctgttccgtctcaaacttggttcgg – (TAMRA)
HER1
Forward Primer tccccgtaattatgtggtgacagatc 250
Reverse Primer acccctaaatgccaccggc
Probe (6FAM) – cagctatgagatggaggaagacggcgt – (TAMRA)
HER2
Forward Primer caaccaagtgaggcaggtcc 101
Reverse Primer ggtctccattgtctagcacgg
Probe (6FAM) – agaggctgcggattgtgcga – (TAMRA)
HER3
Forward Primer gggagccgcttccagact 98
Reverse Primer ttgaggccggtgatcagaaa
Probe (6FAM) – tggactcgagcaacattgatggatttgt – (TAMRA)
HER4
Forward Primer tgttcggaacccatggcct 167
Reverse Primer agcatctgccgtcacattgttct
Probe (6FAM) – atggtagttcaggatgtggacgttgcca – (TAMRA)
MMP9
Forward Primer ccctggagacctgagaacca 77
Reverse Primer cccgagtgtaaccatagcgg
Probe (6FAM) – attcctctgccagctgcctgtcg – (TAMRA)
Muc-1
Forward Primer gtgccccctagcagtaccg 123
Reverse Primer gacgtgcccctacaagttgg
Probe (6FAM) – cattacctgcagaaaccttctcataggggct – (TAMRA)
HPV16-E6
Forward Primer aatgtttcaggacccacagg 124
Reverse Primer ctcacgtcgcagtaactgttg
Probe (6FAM) – cgacccagaaagtt – (TAMRA)
HPV16-E7
Forward Primer caagcagaaccggacaga 96
Reverse Primer gtctacgtgtgtgctttgta
Probe (ROX) – caagtgtgactctacgcttcggttgtg – (BHQ-2)
TBP
Forward Primer cacgaaccacggcactgatt 89
Reverse Primer ttttcttgctgccagtctggac
Probe (VIC) – tcttcactcttggctcctgtgcaca – (TAMRA)
uPA
Forward Primer actgcaggaacccagacaacc 70
Reverse Primer tggacaagcggctttaggc
Probe (6FAM) – ctgcacatagcaccagggtcgcct – (TAMRA)
VEGF-C
Forward Primer tcaaggacagaagagactataaaatttgc 137
Reverse Primer actccaaactccttccccacat
Probe (6FAM) – atacacacctcccgtggcatgcattg – (TAMRA)
VEGF
Forward Primer gcacccatggcagaagg 90
Reverse Primer ctcgattggatggcagtagct
Probe (6FAM) – ctgatagacatccatgaacttcaccacttcgt – (TAMRA)