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. 2018 Apr 24;9(31):21876–21892. doi: 10.18632/oncotarget.25082

Figure 4. Attenuation of tumor growth by SIK2 is accompanied with simultaneous inhibition of MAPK and PI3K/Akt signaling pathways.

Figure 4

MDA-MB-231 cells overexpressing SIK2 were injected subcutaneously to left flanks of SCID mice while the right flanks received empty vector transfected or parental controls (n=6 mice per group). (A) Representative images of tumors excised 5 weeks post injections were shown. Bar graphs represent mean values of the weights and volumes of tumors (± SD). (B) Lysates from excised tumors were subjected to Western blotting. Blots were probed with the indicated antibodies. Band intensities of SIK2 were normalized to that of β-actin, p-ERK to that of ERK and p-Akt to that pan-Akt; mean values of three independent experiments are presented as bar graphs (± SD). (C) Tumor sections (6 sections / tumor) were stained with anti-Ki67 or anti-active caspase-3, and nuclei were visualized by DAPI. The fractions of Ki67 or active caspase-3 positive cells were graphically represented. *P≤0.05 and the Scale bar: 50 um.