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. 2018 Apr 24;9(31):21731–21743. doi: 10.18632/oncotarget.24932

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Copyright: © 2018 Pavon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A, B) Stereotaxic implantation of EPN cells in brain tumor experimental models. (C) Drawing based on Swanson’s Stereotaxic Atlas Guidelines for the indicated coordinates from Bregma -3.80 mm (region of infusion into EPN cells). (D) Representative sagittal section demonstrating the injection of MION-Rh labeled EPN cells into the third ventricle. (F) Combined fluorescence and X-ray tomography for in vivo detection of EPN cells. F´) Fluorescence image detail. (E, G) MRI monitoring of EPN cells and MION-Rh detection. (H) Radiofrequency bobine of animal positioning. I) MRI equipment for small rodent neuroimaging: 2 Tesla superconducting magnet 85310HR.