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. 2018 Apr 24;9(31):21731–21743. doi: 10.18632/oncotarget.24932

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Copyright: © 2018 Pavon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The xenograft tumors displayed characteristic histological features of the original anaplastic EPN, describing the following tests: Immunohistochemical xenograft tumors (EPN1-5) analysis with Prussian Blue for MION-Rh detection, immunohistochemical assay of tumor for glial fibrillary acidic protein (GFAP), hematoxylin and eosin staining, immunohistochemical assay of tumor for Ki67 to detect cell proliferation, vimentin (VIM) to intermediate filament, Nestin to neuron precursor and synaptophysin (SYP) to neuron mature. Black circle: arrangement pseudorosettes with classic intracellular lumens; black arrow: EPN cells that proliferate in the ependymal channel light; white arrow: EPN cells that invade in surrounding region; yellow arrow: hemosiderin granules.