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. 2018 Apr 24;9(31):21950–21964. doi: 10.18632/oncotarget.25134

Figure 4. Ligand-independent Wnt-signaling activation in CRC-TIC spheroid lines.

Figure 4

(A, B) Expression levels of MKI67 (Ki-67) (A) and AXIN2 (B) mRNAs. Normal colonic epithelial SC spheroids were cultured in the eL-WRN medium (Normal stem) or the differentiation medium (Normal diff), whereas CRC-TIC spheroids were cultured in the cancer medium supplemented with EGF and bFGF. Shown are the mean expression levels in triplicates relative to those in normal colonic epithelial SCs (Normal stem). Data of three slow-growing CRC-TIC lines are presented in red.