Table 4. Virulence genes primer used in this study and the protein functions.

Virulence gene	Nucleotide sequence	Protein function/description	Reference
bap	F: AGTTAAAGAAGGGCAAGAAG R: GGAGCACCACCTAACTGA	Biofilm maturation, maintenance	[54]
surA1	F: CAATTGGTAGCTGGCGATCA R: TTAGGCGGGACTCAGCTTTT	Surface antigen protein 1	[13]
BasD	F: CTCTTGCATGGCAACACCAC R: CCAACGAGACCGCTTATGGT	Acinetobactin biosynthesis Iron acquisition system	[55, 56]
BauA	F: TGGCAAGGTGAAAATGCACG R: GCCGCATATGCCATCAACTG	Acinetobactin transport Iron acquisition system
Pld	F: CCGTCAATTACGCCAAGCTG R: CTGACGCTACCTGACGGTTT	Phospholipase D	[57, 58]
omp33-36	F: ATTAGCCATGACCGGTGCTC R: CCACCCCAAACATGGTCGTA	Outer membrane porin	[59–61]
paaE	F: CTATTTAGGCGTTGCTGCGG R: CCTTACAACGACAGGTCGCA	phenylalanine catabolic pathway	[62, 63]
pglC	F: TGGATGAGTTAGCTGC R: TTTTACAAATAGTTAAGC	O-glycosylation system capsular polysaccharide	[14, 64]
traT	F: GGTGTGGTGCGATGAGCACAG R: CACGGTTCAGCCATCCCTGAG	Serum-resistance-associated	[65]