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Both Tpk1 and Tpk2 are required for colonization and necrosis in kidneys and brains. Histopathological analyses of mouse kidney and brain organs obtained from C. tropicalis-infected mice at day 3. Organs were fixed in 10% formaldehyde solution, paraffin-embedded, and stained with PAS and H&E to observe C. tropicalis colonization and tissue necrosis, respectively. White arrowheads indicate fungal cells (hyphae or yeasts). Scale bar = 50 µm.
