Figure 2.

Effect of adoptive transfer of naïve splenic (B)cells to Rag1^−/− mice on lung and brain CFU and lymphocyte profiles of C. neoformans-infected mice. Lung (A) and (B) brain CFU are depicted 21 d post-infection on the Y axis for wild-type, Rag1^−/−, and Rag1^−/− (Rag1^−/− + B cells) mice that received 10⁶ naïve splenic B cells i.v. one week before infection, depicted on the X axis. Lung lymphocyte numbers of B-1a and B-1b B (C) and B-2 B and T (D) cells determined by flow cytometry are depicted for wild-type and Rag1^−/− + B cells mice 21 d post-C. neoformans infection (28 d after adoptive transfer). Each symbol represents one mouse; (A-B) present data from 3 separate experiments, (C-D) represent the results of one experiment. (C-D) Figure legend applies to both panels. Bars are medians. **p < 0.01, ***p < 0.001, Kruskall-Wallis test, correcting for multiple comparisons; ns – not significant