Figure 7.

ASN100 prevents Hla-mediated tissue damage in a human 3D lung tissue infection model. EpiAirway™ tissues were infected with S. aureus TCH1516 at MOI 100 for 24 hours in the presence of control mAb, ASN-1, ASN-2 or ASN-100 (2 µM each). A: H&E staining of tissues. B: LDH release measured from basolateral compartment. C: LDH release measured with wild-type and gene deletion mutant TCH1516 strains in absence of mAbs. D: Relative increase in bacterial CFUs determined in the apical compartment. E: Viability of human neutrophils exposed to toxins produced in the control mAb sample in the presence of ASN-1, ASN-2 and ASN100 (1 µM each). Error bars indicate mean +/− SEM from duplicate measurements.