Figure 3.

Bm-wt LPS preferentially triggered maturation of FL-DCs in vitro, while Bm-wadC LPS activated that of both GM-DC and FL-DC subsets. FL-DCs were non-treated (Mock) or stimulated for 24 h with Bm-wt LPS, Bm-wadC LPS, Ochrobactrum anthropi 331 LPS, Yersinia enterocolitica O:9 LPS or E. coli LPS. Brucella LPSs, Y. enterocolitica and O. anthropi LPS were used at the concentration of 10 μg/mL and E. coli LPS was at 100 ng/mL. (A) MHCII and co-stimulatory molecule expression levels (MFI, Mean of Fluorescence Intensity) were measured by flow cytometry. (B) Cytokine secretion was determined in whole FL-DC culture supernatants by ELISA. The graphs show combined data from at least three independent experiments. All error bars are standard deviations obtained from pooled data. Significant differences from mock only (A) or from mock or from Bm-wt LPS (B) are shown. *, P < 0.05; **, P < 0.001; ***, P < 0.0001; ****, P < 0.00001. ns, non-significant.