Skip to main content
. 2018 Feb 27;9(1):318–330. doi: 10.1080/21505594.2017.1414134

Figure 5.

Figure 5.

Val1686 induced-apoptosis depends on caspases activation. (A) Measurement of caspase-8, -9 and -10 activity. FHM cells were either uninfected (Ctrl), or infected with wild-type ZJO (WT), Δval1686 or ΔvscC. caspase-8, -9 and -10 activity assays were conducted after 2 h infection. (B) Inhibitory effect of three caspase inhibitors on V. alginolyticus-induced apoptosis. FHM cells were pre-incubated for 1 h with 20 μM of caspase-8 inhibitor (Q-IETD-Oph), caspase-9 inhibitor (Q-LEHD-Oph) and negative control inhibitor (Q-VE-Oph) and then infected with wild-type ZJO. Inhibitory effects on apoptosis were evaluated using the caspase-3 activity assay. (C) Inhibitory effect of three caspase inhibitors on caspase-8 and caspase-9 activity. FHM cells were treated as in (A) and measured for caspase-8 and -9 activity. The data are expressed as fold-increase compared to the corresponding values of caspase activity in uninfected cells (Ctrl) (3 independent experiments). A Kruskal Wallis test followed by a Tukey-Kramer multiple comparisons test versus control group (* P < 0.05, *** P < 0.001).