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Color change in the presence of Qo-site inhibitors. A) Stigmatellin blocks all proton release by tightly binding ISPH and preventing its oxidation. B) Azoxystrobin also prevents Qo-site turnover, but proton release from oxidation of ISPH still occurs. C) Although net proton release is always observed in the presence of azoxystrobin, at higher pH values there is also an unexpected initial uptake of protons. (Flashes indicated by arrows on time axis.)
