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. 2018 May 1;9(33):23126–23148. doi: 10.18632/oncotarget.25226

Figure 9. IL-34 differentially regulates migration and signaling of human breast cancer cell lines.

Figure 9

(A) Comparison of gene expression in MCF7, SK-BR-3, and MDA-MB-231 breast cancer cells with THP-1 macrophages. Graphs show results of qRT-PCR for IL-34, CSF-1, CSF-1R, PTPRZ1 and SDC1 performed on RNA from human MCF7, SK-BR-3 and MDA-MB-231 breast cancer cells as well as THP-1 macrophages. (B) Quantification of migrated MCF7, SK-BR-3 and MDA-MB-231 breast cancer cells from an in vitro migration assay are shown. Cells were either left unstimulated (control) or stimulated with IL-34, CSF-1 or pretreated with CSF1-R blocking antibody (antiCSF1R) prior to cytokine treatment. *, p < 0.05 vs. control;, p < 0.05 vs recCSF1. (C) Differential regulation of signaling upon IL-34 or CSF-1 treatment in human breast cancer cell lines. MCF7, SK-BR-3, and MDA-MB-231 breast cancer cells were stimulated with recombinant IL-34 or CSF-1 protein for the indicated times. Western blot images of indicated proteins in breast cancer cells are shown. p- indicates phosphorylated proteins.