Figure 3. STn⁺ and CD133⁺ cells display chemoresistance.

(A) OV90, OVCAR3 and OVCAR4 cells were cultured for 72 hours in the presence of either vehicle control or carboplatin (10 μM). Following treatment, adherent cells were trypsinized and analyzed by flow cytometry to determine the relative frequency of STn^-/CD133^-, STn⁺/CD133^-, STn^-/CD133⁺ and STn⁺/CD133⁺ sub-populations. (B) In a parallel analysis, the total number of live, apoptotic and necrotic cells remaining after 72 hours of exposure to vehicle control or carboplatin (10 μM) was determined by annexin assay. Error bars represent the mean ± SEM. ^*p < 0.05. All experiments were repeated a minimum of three times.