Skip to main content
. 2018 May 1;9(33):23289–23305. doi: 10.18632/oncotarget.25289

Figure 5. S3F-CL-MMAE decreases CSC frequency in vitro.

Figure 5

The extreme limiting dilution assay was used as surrogate to determine the effect of S3F-CL-MMAE on CSC frequency. Seventy two (A) and 144 hours (B) following treatment with vehicle or 10 nM S3F-CL-MMAE, OV90, OVCAR3 and OVCAR4 cells (1-10-20 or 40/well) were plated under tumorsphere culture conditions and the number of spheres that formed after 7-8 days in culture was determined. Stem cell frequencies were calculated as described in Materials and Methods. Error bars represent the SEM. *p < 0.05. All experiments were repeated four times.