Figure 6. Treatment with the anti-STn, S3F-CL-MMAE, on CD133 and STn populations in vitro.

OV90, OVCAR3 and OVCAR4 cell lines were harvested 72 (A) or 144 (B) hours post treatment with vehicle or S3F-CL-MMAE (10 nM), and the relative frequency of STn^-/CD133^-, STn⁺/CD133^-, STn^-/CD133⁺ and STn⁺/CD133⁺ fractions was determined by flow cytometry. Error bars represent the mean and SEM. ^*p < 0.05. All experiments were repeated four times.