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. 2018 May 1;9(33):23264–23273. doi: 10.18632/oncotarget.25297

Figure 2. Knockdown of K8 enhances TRAIL induced apoptosis.

Figure 2

(A-C) Cells were transfected with a control siRNA or siRNA specific to KRT8 transcript for 72 hours, followed by TRAIL stimulation (100 ng/ml [T47D and BT474] or 150 ng/ml [MCF7]) for 24 hours. The resultant cells were analyzed by immunoblotting using antibodies specific to K8/K18, DR4, DR5, caspase-3, caspase-8, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Caspase activation was identified by decrease in pro-enzyme form (ProC-8 and ProC-3). (D-F) Cells were treated as above and analyzed by flow cytometry after staining with Annexin-V-FITC and propidium iodide (PI).