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. 2018 May 1;9(33):23253–23263. doi: 10.18632/oncotarget.25298

Figure 1. MMSET upregulation promotes sphere formation and invasion in EC cells.

Figure 1

(A) qPCR analysis of MMSET mRNA expression in the immortalized human endometrial epithelial EM cell line and EC cell lines. (B and C) qPCR analysis and western blotting analysis of MMSET expression in Ishikawa cells after transient overexpression of MMSET, and in HEC-1 cells after transient knockdown of MMSET. (D and E) Sphere formation (D) and invasion (E) in Ishikawa cells after transient overexpression of MMSET. (F and G) Sphere formation (F) and invasion (G) in HEC-1 cells after transient knockdown of MMSET. (H) Western blotting analysis of indicated proteins in Ishikawa and HEC-1 cells after overexpression or knockdown of MMSET. (I) Ishikawa cells were transfected with MMSET expression vector or the control vector in culture plates. 48 hours later, MMSET-overexpressing Ishikawa cells were injected into nude mice. Tumor volume and weight were measured. (J) HEC-1 cells were transfected with MMSET siRNA or the control siRNA in culture plates. 48 hours later, MMSET-knockdown HEC-1 cells were injected into nude mice. Tumor volume and weight were shown. **P < 0.01.