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. 2018 May 1;9(33):23253–23263. doi: 10.18632/oncotarget.25298

Figure 2. Twist1 mediates MMSET-induced sphere formation and invasion.

Figure 2

(A) Ishikawa cells were transfected with control vector or MMSET-expressing vector, together with siRNA targeting MMSET or control siRNA. Relative Twist1 expression was analyzed using qPCR. (B) Morphological appearance of cells described in (A) was analyzed by microscopy. (C and D) Invasion (C) and sphere formation (D) of cells described in (A) was determined using transwell invasion and sphere formation assay. (E) Western blotting analysis of indicated proteins in cells described in (A). **P < 0.01.