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. 2018 May 1;9(33):22929–22944. doi: 10.18632/oncotarget.25149

Figure 4. SAP155 (SF3b1) was required for cyclin E and Notch 1 mRNA expression in ESCC cells.

Figure 4

SAP155 (SF3b1) siRNA suppressed cyclin E, TP53, Notch 1, FIRs, TP53, GSK3β, FBW7, E-cadherin, PP1, ERK and c-Myc expression in esophageal cancer cells, YES2 and YES3. (A) SAP155 siRNA decreased cyclin E, Notch1 FIRs and TP53 expression in YES2 (pathogenic TP53), but observed less effective in YES3 cells (truncated TP53 expression). FBW7 expression was irrelevant to SAP155 siRNA in YES2 and YES3 cells. Note, GSK3-β was suppressed by SAP155 siRNA in YES2 cells. FBW7, E-cadherin, PP1, ERK and c-Myc were not affected by SAP155 siRNA in YES2 and YES3 cells. (B) qRT-PCR of Notch1 mRNAby SAP155 siRNAin YES2 and YES3. (C) qRT-PCR of cyclin E mRNA by SAP155 siRNAin YES2 and YES3. (D) SAP155 siRNA decreased Notch1 and TP53 expression in HeLa cells (benign TP53).