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. 2018 Apr 24;9(1):783–803. doi: 10.1080/21505594.2018.1449507

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The nuclear import of M protein and the replication of NDV are not inhibited by ivermectin. (A) The subcellular localization of NDV M protein in virus-infected DF-1 cells treated with ivermectin or DMSO at 12 hpi. DAPI was used to stain nuclei. Original magnification was 1 × 200. (B) The intracellular distribution of NDV M protein obtained from (A) was detected by Western blotting. Lamin B1 for the nucleus and tubulin for the cytoplasm were used as cellular markers. N represents the nucleus and C represents the cytoplasm. (C) The CPE in virus-infected DF-1 cells treated with ivermectin or DMSO at 12 hpi was observed under phase-contrast microscope. Original magnification was 1 × 200. (D) The expression of GFP in virus-infected DF-1 cells was observed under fluorescence microscope and detected by Western blotting. (E) DF-1 cells were treated with the drug ivermectin or DMSO and then infected with rSS1GFP at an MOI of 0.1. The cell culture supernatants were collected at 12 and 24 hpi, and the virus titers were determined as TCID₅₀ in DF-1 cells.