Table 2.
Bacterial strains and plasmids used in this work.
| Strain or plasmid | Relevant characteristics | Sources or references |
|---|---|---|
| STRAINS | ||
| A.baumannii | ||
| ATCC 17978 | Clinical isolate | ATCC |
| Δ0242 | A1S_0242 gene deletion mutant obtained from the ATCC 17978 strain | This study |
| ATCC 17978 + pWH1266-Km | ATCC 17978 harboring the empty pWH1266-Km plasmid; KmR, TetR | This study |
| Δ0242 + pWH1266-Km | Δ0242 harboring the empty pWH1266-Km plasmid; KmR, TetR | This study |
| Δ0242 complemented | Δ0242 harboring the pWH1266-Km-0242 plasmid; KmR | This study |
| Δ3850 | A1S_3850 gene deletion mutant obtained from the ATCC 17978 strain | This study |
| Δ3850 + pWH1266-Km | Δ3850 harboring the empty pWH1266-Km plasmid; KmR, TetR | This study |
| Δ3850 complemented | Δ3850 harboring the pWH1266-Km-3850 plasmid; KmR | This study |
| Δ0242/Δ3850 | A1S_0242 and A1S_3850 genes deletion double mutant obtained from the ATCC 17978 strain | This study |
| E. coli | ||
| TG1 | Used for DNA recombinant methods | Lucigen |
| PLASMIDS | ||
| pWH1266-Km | A. baumannii shuttle vector; KmR, TetR | Álvarez-Fraga et al. 2016 [68] |
| pWH1266-Km-0242 | pWH1266-Km harboring the A1S_0242 gene; KmR | This study |
| pWH1266-Km-3850 | pWH1266-Km harboring the A1S_3850 gene; KmR | This study |
| pMo130 | Suicide vector for the construction of A. baumannii isogenic derivative; KmR, SacB, XylE | Hamad et al. 2009 [69] |
KmR: kanamycin resistance. TetR: tetracycline resistance.