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. 2018 May 4;9(1):818–836. doi: 10.1080/21505594.2018.1451184

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — T98G glioblastoma cells internalization of EVs from A. castellanii. EVs were stained with DiI C18 (red-stained) and incubated with the CHO for different time points. T98G nuclei were stained with DAPI (blue) and the CtxB- Alexa 488 (green) was used to stain the GM1 ganglioside, a lipid raft marker located on the cell membrane. A. castellanii EVs co-localized with the lipid rafts, suggesting association of GM1 on the T98G internalization of EVs. At early time points, EVs are associated to the membrane of T98G cells; at 1 h, EVs seems to be randomly distributed through the cytoplasm of T98G cells. Results are representative of at least 10 different fields.