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Binding of SCIN to S. aureus cells increases upon incubation in serum. Western blotting analysis of S. aureus Newman ΔspaΔsbi cells collected by centrifugation (P) and growth medium fractions (S) using 6D4–800CW. The presence or absence of C3 convertases due to serum incubation, and the addition or absence of SCIN are indicated with + or -, respectively.
