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Disruption of 112092 and 108920 genes. (A) Targeted replacement strategy using a vector generated by fusion PCR with the pyrG gene as selective marker. Black arrowheads indicate the primer pairs used for amplification of DNA fragments. Probes are indicated (dashed bar). (B) Southern analysis of gDNAs from M. circinelloides CBS277.49 and transformants. DNAs were digested with EcoR V and Sma I to detect 112092 and 108920 gene deletions, respectively.
