Table 3.

Genes coding for extracellular enzymes identified in the genome comparison study among CBS277.49 and NRRL3631 genomes as absent, or discontiguous sequences in NRRL3631. ^aNumber of duplicated genes in CBS277.49 M. circinelloides genome based upon BLAST with E-value < E⁻⁵⁰ and ≥ 70% identity. ^bDistribution of orthologues within the fungi species based upon BLAST with E-value < E⁻²² and ≥ 50% identity. Ac: Absidia glauca, Cc: Choanephora cucurbitarum, Cg: Colletotrichum gloeosporioides, Lc: Lichtheimia corymbifera Lr: Lichtheimia ramosa, Ma: Mucor ambiguos, Mc: Mucor circinelloides, Me: Morteriella elongata, Pb: Phycomyces blaskespeanus, Pp: Parasitella parasítica, Rm: Rhizopus microsporus, Ri: Rhizopus irregularis, Rd: Rhizopus delemar, Ro; Rhizopus oryzae, Um: Ustilago maydis.

Protein ID	KOG ID	Protein function	No. of Paraloguesa	Fungal taxonomic distribution of best hitsb
Unique genes in CBSS277.49
167922	KOG2126	Glycosylphosphatidylinositol anchor synthesis protein	—	Mc, Ma, Pp, Cc, Rm
167058	KOG1216	von Willebrand factor, related coagulation proteins	—	Mc, Pp, Cc, Ma, Rd, Rm, Lc
115405	KOG3599	Ca2+ cation channel polycystin, Mucine	—	Mc
112425	KOG1218	Proteins containing Ca2+-binding EGF-like domains	—	Mc, Ma, Pp
76897	KOG2806	Chitinase	—	Mc
185052	—	Putative glycoside hydrolase/deacetylase,	2	Mc, Ma, Pp, Rm, Pb
163412	—	Chitin binding protein, putative peritrophin-A	2	Mc
112072	—	Chitin binding protein, putative peritrophin-A	1	Mc, Ma
113101	—	Chitin binding protein, putative peritrophin-A	1	Mc
110615	—	Putative MFS general substrate transporter	1	Mc, Ma, Pp, Cc
80244	—	Putative membrane transporter	—	Mc, Pp
189787	—	Unknown	—	Mc, Cc, Lc, Pb
155646	—	Unknown	2	Mc, Ma Pp, Rd Cc, Ac, Pb, Lr, Lc
104593	—	Unknown	—	Mc
83937	—	Unknown	—	Mc
79369	—	Unknown	—	Mc
77057	—	Unknown	2	Mc, Ma, Rd, Rm, Fo, Af
38654	—	Unknown	1	Mc, Ma, Cc, Lc, Pp, Ac, Rm, Rd
Discontiguos genes in NRRL3631
155853	KOG4157	β-1, 6-N-acetylglucosaminyltransferase	—	Mc, Pp, Rd, Cc, Rm
155032	KOG3083	Prohibitin	1	Mc, Pp, Rd, Ma, Rm, Cc, Ag, Pb, Me, Lc, Pp Pb, Ri, Cg, Um
141273	KOG1285	β-carotene 15-dioxygenase	—	Mc, Ma, Pp, Rd, Rm, Ro, Rm, Ag
108920	KOG2410	hypothetical γ-glutamyltransferase	2	Mc, Pp, Rm,Ma, Pb, Ag, Cc, Rd
83381	KOG1550	Extracellular protein SEL-1	—	Mc, Pp, Cc, Rm, Pb, Ag, Lr
39631	KOG3339	Predicted glycosyltransferase Alg14	—	Mc, Pp, Cc, Pb, Lc, Ag, Ri
106371	—	Unknown	2	Mc, Ma, Pp, Pb, Lr, Rm, Lc
112092	—	Unknown	—	Mc, Pp, Ag
116342	—	Unknown	—	Mc, Ma, Pp
166851	—	Unknown	4	Mc, Ma, Rm, Rd