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. 2018 Feb 27;9(1):604–620. doi: 10.1080/21505594.2017.1417723

Figure 8.

Figure 8.

Immunization with D-alanine auxotrophic vaccine elicits cross reactive antibodies and protects against staphylococcal infections. A. Cross reactive IgG antibody titers induced after vaccination against different staphylococcal strains: S. aureus 132 Δspa, USA300 LAC, ED98 (from poultry), ED133 (ovine origin) and RF122 (bovine origin). Serum was obtained from BALB/c mice (n = 5) two weeks after vaccination with two doses of S. aureus 132 Δdat Δalr1 Δalr2 (about 1.7 × 108 CFU). The boxes represent the first and third quartiles; the horizontal line represents the median; the whiskers represent the interquartile range. *P < 0.05 (Mann-Whitney U test,) compared with saline group. B-E. Mice survival (C and E) and percentage of body weight loss (B and D) after vaccination with S. aureus 132 Δdat Δalr1 Δalr2 (two dose-schedule, approximatively 3 × 107 CFU) or saline administration (n = 6/group), and challenge with a lethal dose of USA300 LAC (3 × 107 CFU) (B and C) or RF122 (3.5 × 107 CFU) (D and E). *P < 0.05 and **P < 0.005 (Log rank Mantel-Cox test), compared with saline group.