Table 2.

Primers used in gene expression analysis of selected genes of D. citri by real time RT-PCR^a.

Gene	NCBI Reference Sequence		Primer (Forward and Reverse)	TM (˚C)	Product size (bp)
DcTPS -1	XM_017448460.1	F	AGTCATTATCCAGGGCAACG	59.96	198
		R	CAGAAGGTGCCATTACAGCA	59.86
DcTPS -2	XM_008470475.1	F	CCTACATCCGGATTGACCAG	60.33	192
		R	CGTACAGTTGGCGAATTCCT	60.13
DcTREH -1	XM_008470849.1	F	GGTAATGGAGGCGAGTACGA	60.10	190
		R	ACAGGAACAGGATGCCTCAC	60.12
DcTREH -2	XM_008470850.2	F	TCACCTCCAACCCGAGTATC	59.93	191
		R	CAGTTCTCGAAACCGTCCTC	59.84
DcTREH -3	XM_008476679.2	F	CTGAAGTGTGTGCTCCGAAA	60.02	197
		R	GAGTCGGGAAACAGTGAAGC	59.85
DcTREH -4	XM_008476680.2	F	TGCAGTTCAGATGGCTTCAC	59.99	198
		R	GGCGACCAGTCTTCAAACTC	59.85
DcTREH -5	XM_008476684.2	F	GCAGTGGGACTACCCTAACG	59.62	195
		R	CCGTGTCCTCCTGGTACTGT	60.03
DcER -1b	XM_008489926.2	F	GAGAGGTGAAACAGGCGGTA	60.25	200
		R	TGCAGGTTTCACGAGATCAG	59.98
DcTKT -1	XM_008486923.2	F	CCACGCACAGAGACATCATT	59.71	201
		R	CGGTGGTCGGTACAGATTTT	59.85
DcTKT -2	XM_008471702.2	F	CCAGATCCGTATGGGAGCTA	60.05	201
		R	CCTGGGATTGTACGGAACAT	59.67
DcMPI	XM_008474470.2	F	CCAGAATTGGCCATAGCACT	60.10	199
		R	GAGCTTGGGGAGCACTCATA	60.36
DcHK	XM_008477526.2	F	AAGGATAGACCGGGATGACA	59.36	199
		R	CTGGAAAGCGCCTAGTTTCA	60.51
DcHK2 -1	XM_008480214.1	F	GCGAGCTGGTTCGTGTAGTA	59.10	200
		R	ATCCACCGCTATGGTGATGT	60.23
DcHK2 -2	XM_017444207.1	F	CTAGCCGGCCTAGGATTACC	60.08	202
		R	TCATCAGGTCATGGAAGTGC	59.64
DcProDH	XM_017446986.1	F	GAAGGTGACCAAGGATGCTC	59.66	199
		R	TCGGACCAGGGTGTTTAGTC	59.97
DcCS	XM_008470046.1	F	TGCCCGATGACTCCTTATTC	60.04	190
		R	CCAAGAGCTCTGGACACTCC	59.99
DcPC	XM_008478621.1	F	GAAGCTTTGGCTTCATTTGG	59.82	201
		R	GTCGCGGACACTTACACTCA	59.90
DcSHMT	XM_008473310.2	F	TCGAACCTCATGGGAGAATC	60.01	197
		R	TCCTTTGTGAGTGGTGGTCA	60.13
DcPSPH-1	XM_008483178.2	F	GGCCAAAGTCATCGAGAGTC	59.81	202
		R	GACCACGTTGCCTCCATAAC	60.38
DcPSPH-2	XM_008472446.2	F	TGAACCCGGAGCAGATATTC	60.04	209
		R	ACTCAGGCTCGGCTTCATAA	59.98
α-Tubulinc	XM_008482608.2	F	CTTTCCAACACCACCGCTAT	59.99	196
		R	CTCCTTCTCCAGCCTCCTCT	60.09
Actinc	XM_008470468.2	F	TATCCCAGCCCTGAGCTAGA	59.93	204
		R	CACCATGACACCCTGATGAC	59.80

^aThe listed genes were selected depending on the affected cycles (increase or decrease in its compounds). Furthermore, these genes were assembled based on recent available data in national center for biotechnology information website (NCBI, http://www.ncbi.nlm.nih.gov/gene/).

^bDcER gene has been matched as Diaphorina citri aldose reductase-like (DcAR) using the protein-protein BLAST, based on recent available data in national center for biotechnology information website (NCBI, http://www.ncbi.nlm.nih.gov/gene/).

^cGenes have been used as a reference genes for data normalization according to Tiwari et al., 2011⁴⁸ and Killiny et al., 2014⁴⁹

Abbreviations:

DcTPS-1: Diaphorina citri-α, α Trehalose phosphate synthase;

DcTPS-2: Diaphorina citri-Trehalose phosphate synthase-like;

DcTREH: Diaphorina citri-Trehalase;

DcER: Diaphorina citri-Erythrose reductase;

DcTKT: Diaphorina citri-Transketolase-like;

DcMPI: Diaphorina citri-Mannose-6-phosphate isomerase;

DcHK: Diaphorina citri-Hexokinase-like;

DcHK2: Diaphorina citri-Hexokinase-2-like;

DcProDH: Diaphorina citri-proline dehydrogenase 1;

DcCS: Diaphorina citri-probable citrate synthase 2;

DcPC: Diaphorina citri-pyruvate carboxylase, mitochondrial-like;

DcSHMT: Diaphorina citri-serine hydroxymethyltransferase, cytosolic;

DcPSPH: Diaphorina citri-phosphoserine phosphatase-like