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. 2018 Feb 27;9(1):480–493. doi: 10.1080/21505594.2017.1419116

Table 4.

Molecular methods applied to identify Brevundimonas spp. [45].

Method Target Sequence Species
Real Time PCR gyrB  Forward Primer Brevundimonas diminuta
    ATCGAGATCATGCTGCACTATGAGGG  
    Reverse Primer  
    TGTTGTTGGTGAAGCACAGCATGG  
    Real-Time Probe  
    ACGTCATCGTCATTCGCGGCCAGAA  
Real Time PCR rpoD  Forward Primer AGTTCCTCAAGGCCTATTTCGGCT Brevundimonas diminuta
    Reverse Primer  
    GGCTTCATTCTCGCTGAACTTGGT  
    Real-Time Probe  
    AGCGCATCAAGGAGATGGGCGT  
FISH gyrB  AAGAAGCACAGCGTCCGCTTCGAGC Brevundimonas diminuta
FISH rpoD  TCAAGGCCTATTTCGGCTCGGAGAT Brevundimonas diminuta