Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 15;9(1):898–918. doi: 10.1080/21505594.2018.1460979

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 9. — Characterization of H. suis binding glycosphingolipids from pig stomach. A. Thin-layer chromatogram after detection with anisaldehyde B. Autoradiogram obtained by binding of ³⁵S-labeled H. suis (HS5) at pH 7.2. C. Autoradiogram obtained by binding of ³⁵S-labeled H. pylori strain J99 wt at pH 7.2. The glycosphingolipids were separated on aluminum-backed silica gel plates, using chloroform/methanol/water 60:35:8 (by volume) as solvent system. Lanes 1–4 were total non-acid glycosphingolipids isolated from the stomach of four individual pigs, 40 µg/lane. D-G. LC-ESI/MS of the oligosaccharides obtained by digestion of the non-acid glycosphingolipid fraction of pig stomach (shown in chart A, lane 2) with Rhodococcus endoglycoceramidase II. D. Base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from the non-acid glycosphingolipid fraction of pig stomach. E. MS² spectrum of the ion at m/z 706 (retention time 14.2 min). F. MS² spectrum of the ion at m/z 706 (retention time 18.3 min). G. MS² spectrum of the ion at m/z 706 (retention time 18.5 min). H. Interpretation formulas showing the deduced oligosaccharide structures. Gb3, Galα4Galβ4Glc; Gb4, GalNAcβ3Galα4Galβ4Glc; A6-1, GalNAcα3(Fucα2)Galβ3GlcNAcβ3Galβ4Glc; Le^y-6, Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4Glc; H5-1, Fucα2Galβ3GlcNAcβ3Galβ4Glc; L4, Galβ3GlcNAcβ3Galβ4Glc; nL4, Galβ4GlcNAcβ3Galβ4Glc; H5-2, Fucα2Galβ4GlcNAcβ3Galβ4Glc; Galα3-nLc4, Galα3Galβ4GlcNAcβ3Galβ4Glc; x₂, GalNAcβ3Galβ4GlcNAcβ3Galβ4Glc; Galα3-nLc4, Galα3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc; GbA, GalNAcα3(Fucα2Galβ3GalNAcβ3Galα4Galβ4Glc.