Table 3. Variation between replicate, extraction, assay run, and laboratory observed during repeatability and reproducibility studies.

		Replicate	Extraction	Assay Run	Laboratory
LN34 Assay	CV	0.35 ± 0.06%	0.72 ± 0.27%	1.33 ± 0.32%	9.13 ± 2.06%
	SD	0.09 ± 0.02	0.12 ± 0.07	0.36 ± 0.16	1.89 ± 0.45
	ΔCt	0.18 ± 0.04	0.39 ± 0.12	0.99 ± 0.43	3.70 ± 0.94
β-Actin Assay	CV	0.48 ± 0.13%	2.15 ± 0.75%	1.86 ± 0.70%	6.56 ± 1.00%
	SD	0.10 ± 0.03	0.44 ± 0.12	0.41 ± 0.10	1.34 ± 0.20
	ΔCt	0.18 ± 0.05	0.97 ± 0.28	1.14 ± 0.41	2.57 ± 0.39

Variability shown as average coefficient of variation (CV), standard deviation (SD) and difference in Ct values (ΔCt) with 95% confidence intervals.