Table 4. Tetra-primer ARMS PCR mixtures.

Components	Volume (μL)
ddH2O	1.4
2×GC buffer	10.0
dNTP (2.5 mM each)	2.4
Dimethylsulphoxide	0.8
FO13 (5 μM)	1.6
RO13 (5 μM)	1.2
FI16 (5 μM)	0.4
RI16 (5 μM)	1.6
Template (gDNA; 50 ng/μL)	0.4
Ex Taq (5 U/μL)	0.2
Total volume	20.0