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. 2018 May 4;12(5):e0006473. doi: 10.1371/journal.pntd.0006473

Fig 2. Caveolae/raft-mediated endocytosis is the main route involved in E. granulosus AgB internalization by A549 and RH cells.

Fig 2

Indicated cells were incubated in medium alone (Ctrl), with 100 μg/ml genistein (GNT), or with 5 μg/ml chlorpromazine (CPZ) for 30 min and then exposed to 40 μg/ml AgB for 1.5 h. AgB was detected using anti-AgB polyclonal antibodies followed by anti-rabbit IgG Alexa Fluor 488 conjugated antibody (green). Cell nuclei were labelled with DAPI (blue). A and B, Inhibition of caveolae/raft-mediated endocytosis by genistein reduces AgB internalization. C and D, inhibition of clathrin-mediated endocytosis pathway by chlorpromazine does not cause a significant decrease in uptake of AgB. The quantitative data presented in B and D are measurements from three experiments with RH cells and two with A549 cells. Arbitrary units correspond to immunofluorescence intensity calculated as total immunofluorescence in the cell divided by the area of the cell. Error bars indicate SEM. *p = 0.037, ***p = 0.0004 according to Student’s t-test.