Fig 7. Kinetics of zincosome formation in C. albicans.
Cells were incubated overnight in YNB-zinc-dropout medium (SD0) to deplete zincosomes and pulsed with 25 μM ZnSO4 for indicated time points. Cells were then stained with zinquin to probe for zincosomal zinc and the cell wall stained with Concanavalin A conjugated to Alexa-647. Left hand column shows false colour overlay of cell wall (cyan) and zincosomes (magenta). Right hand column shows DIC; Experiment performed three times and representative images shown.