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. 2018 May 16;4(5):eaar4536. doi: 10.1126/sciadv.aar4536

Fig. 2. P. tricornutum ISIP1 knockdown cells are unable to grow on FOB as the sole source of iron.

Fig. 2

(A) P. tricornutum is able to grow on 15 pM Fe′ (black line) and 10 nM FOB (red line) as sole Fe sources in Aquil media. The ISIP1 knockdown (k/d) line shows reduced growth on Fe′ (blue line) and no growth on FOB (green). Error bars denote SDs from three biological replicates. Before the start of the experiment, cultures were depleted of Fe through a 10-day preculture on low-Fe Aquil. (B) Comparison of siderophore uptake by P. tricornutum wild-type (WT) cells (rhombus markers) versus ISIP1 (square) and ISIP2a (triangle) knockdown cell lines.