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. Author manuscript; available in PMC: 2019 Feb 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Jan 24;57(9):2357–2361. doi: 10.1002/anie.201711441

Figure 2.

Figure 2

Photo-capture performance of the psoralen-modified polyacrylamide gel for 100-bp double-stranded DNA (dsDNA). a) Representative fluorescence images indicating the capture capacity of the psoralen-modified gel. DNA targets were visualized using SYBRTM Gold stain after photo-capture. The fluorescence images at indicated UV irradiation durations were taken after electrophoretic wash. Psoralen concentration in the ‘+’ experiments is 3 mM. Target loading: 40 ng/well. Stain: 2x SYBRTM Gold. Ex: 488 nm. Em: 520 nm BP 40 nm. Experimental conditions were the same for all the following experiments unless otherwise specified. b) Capture efficiency (η) measured at various UV irradiation durations for different psoralen concentrations. n = 3 for each data point; error bars: standard deviation. c) The steady-state capture efficiency (η0) versus the psoralen concentration. d) The reaction rate constant (k) versus the psoralen concentration.