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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Hypertension. 2018 Jan 22;71(3):518–529. doi: 10.1161/HYPERTENSIONAHA.117.10503

Figure 3.

Figure 3

STIM2 is sufficient for increasing the level of phosphorylated (p) CREB, STAT3, and AKT and enhancing the nuclear translocation of NFATC2 in PASMCs. A Western blot analyses (a) on pCREB, CREB, pSTAT3, STAT3, pAKT and AKT in vector- and STIM2-YFP-transfected normal PASMCs. Summarized data (b, means±SE, n=3 normal subjects) showing the protein level of pCREB, CREB, pSTAT3, STAT3, pAKT and AKT in vector- and STIM2-YFP-transfected normal PASMC. *P<0.05 vs. Vector. B, Western blot analyses (a) on pCREB, CREB, pSTAT3, STAT3, pAKT and AKT in vector- and STIM2-YFP-transfected normal PASMCs treated without and with EGTA. Summarized data (b, means±SE, n=3 normal subjects) showing the protein level of pCREB, CREB, pSTAT3, STAT3, pAKT and AKT in vector- and STIM2-YFP-transfected normal PASMCs treated without and with EGTA. *P<0.05 vs. Vector and STIM2-YFP+EGTA. C, Representative fluorescent images showing NFATC2 (red), STIM2 (green) and nuclei (blue) in vector- and STIM2-YFP-transfected normal PASMCs treated without and with EGTA. Summarized data (right, means±SE, n=3 normal subjects) showing the percentage of NFATC2-activated cells in vector- and STIM2-YFP-transfected normal PASMCs treated without and with EGTA. ***P<0.001 vs. Vector and STIM2-YFP+EGTA. D, Summarized data (means±SE, n=3 normal subjects) showing the resting [Ca2+]cyt in vector- and STIM2-YFP-transfected normal PASMCs treated without and with EGTA. ***P<0.001 vs. Vector and STIM2-YFP+EGTA.