Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 22;67(2):91–104. doi: 10.1538/expanim.17-0153

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2018 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

PMC Copyright notice

Fig. 5. — Sperm passage through the cumulus cell layer and the ZP. (A) The cumulus-cell oocyte complex (COC). Ovulated eggs are covered by a cumulus cell layer and the zona pellucida (ZP). (B) Sperm passage through the cumulus cell layer. The spermatozoa penetrating through the cumulus cell layer were observed using the red fluorescence localized in the midpiece. The egg is highlighted with a white dotted line. (C) Rescue of ZP penetration failure using glutathione (GSH). When GSH is used, the ZP is destabilized and expanded. Ppp3cc KO spermatozoa can penetrate the ZP in the presence of GSH and fertilize the egg. Black arrows indicate the pronuclei.