Table 6.
Protein Expression in Sciatic Nerve During Early and Late Disease
| 8 Weeks of Age | 24 Weeks of Age | |||||
|---|---|---|---|---|---|---|
| Immune Marker | db/+ | db/db | p-value | db/+ | db/db | p-value |
| Eotaxin (CCL11) | 282.5 ± 61.87 | 382.2 ± 101.6 | 0.132 | 115.1 ± 21.7 | 168.7 ± 60.3 | *0.0379 |
| IFN-γ | 0 | 13.4 ± 12.4 | 0.606 | 0 | 6.4 ± 12.7 | 0.4615 |
| IL-1α | 0 | 10.4 ± 16.5 | 0.4545 | 0 | 5.8 ± 15.2 | 0.9999 |
| IL-1β | 14.3 ± 12.7 | 10.8 ± 12.9 | 0.8052 | 0 | 7.0 16.2 | 0.4615 |
| IL-4 | 0.8 ± 1.6 | 0.8 ± 1.8 | 0.9999 | 0.02 ± 0.1 | 2.0 ± 3.7 | 0.4615 |
| IL-6 | 17.7 ± 28.9 | 39.3 ± 36 | 0.1277 | 0 | 14.4 ± 27.6 | 0.4615 |
| IL-10 | 49.7 ± 8.3 | 97.1 ± 10.7 | **0.0099 | 55.3 ± 12.1 | 80.1 ± 17.6 | ** 0.0099 |
| IL-13 | 0 | 7.882 ± 19.3 | 0.9999 | 0 | 0 | NA |
| IP-10 (CXCL10) | 113.6 ± 19.0 | 124.0 ± 15.4 | 0.4848 | 65.8 ± 12.4 | 85.2 ± 23.2 | 0.1649 |
| KC (CXCL1) | 116.0 ± 23.2 | 98.3 ± 12.2 | 0.3095 | 42.3 ± 7.2 | 42.1 ± 7.6 | 0.9015 |
| MCP-1 | 92.1 ± 33.5 | 103.7 ± 7.8 | 0.3939 | 0.8 ± 0.8 | 2.0 ± 1.9 | 0.6807 |
| MIG (CXCL9) | 144.0 ± 32.1 | 164.5 ± 22.6 | 0.2403 | 79.9 ± 26.6 | 87.5 ± 19.5 | 0.3179 |
| MIP-1α | 42.6 ± 48.5 | 72.4 ± 15.1 | 0.0649 | 0 | 13.3 ± 19.3 | 0.1923 |
| MIP-1β | 36.4 ± 26.9 | 35.5 ± 5.5 | 0.132 | 0 | 4.1 ± 7.3 | 0.4615 |
| RANTES (CCL5) | 0 | 0 | NA | 0 | 0 | NA |
| TNF-α | 0 | 0 | NA | 0 | 3.6 ± 9.5 | 0.9999 |
| MMP-12 | 3.5 ± 6.8 | 334.6 ± 130.5 | **0.0022 | 6.9 ± 8.6 | 38.4 ±14.6 | **0.0012 |
Units = pg cytokine/chemokine per mg total protein lysate; data expressed as mean ± SD; comparisons made using Mann-Whitney,
*
p < 0.05,
**
p < 0.01;
NA = not applicable