Skip to main content
. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: Cancer Res. 2018 Mar 6;78(10):2524–2535. doi: 10.1158/0008-5472.CAN-16-2140

Figure 3. Reduced xenograft growth of CHIP-overexpressing BT474 cells in nude mice.

Figure 3

(A) Tumor volume of CHIP-lo (MSCV-puro) control and CHIP-hi (MSCV-CHIP) BT474 xenografts over time. Mean+/- SEM, N = 6 per group., * = p<0.05, ** = p<0.01, *** = p<0.001. (B) Photograph of resected CHIP-lo (upper) and CHIP-hi (lower) BT474 cell xenografts. (C) Western blotting to visualize the endogenous and overexpressed (Myc-tagged) CHIP in resected BT474 xenografts; HSC70, loading control. (D) Representative H&E staining and mitotic events (red circles) of CHIP-lo (left) and CHIP-hi (right) BT474 xenograft tumor sections. (E) Quantification of mitotic events of sections depicted in D. Mean +/- SEM, n=3. (F) Ki67 (brown) and cleaved caspase 3 (CC3; red) co-staining of CHIP-lo (left) and CHIP-hi (right) BT474 xenograft tumor sections. (G, H) Quantification of Ki67 (G) and CC3 (H) positive cells depicted in F. Mean +/- SD, n=4.