Table 2.
Stereological Parameters for Optical Fractionator.
| Regions of interest | Stereology Objective | Section Interval | Counting Frame (μm) | Grid Spacing (μm) | Dissector Height (μm) | Guard Zone (μm) Above | |
|---|---|---|---|---|---|---|---|
| c-Fos | Dorsal Premotor Cortex | 40X | 1/40 | 250 × 250 | 1200 × 1200 | 15 | 2 |
| Ventral Premotor Cortex | 950 × 950 | ||||||
| Synaptophysin | Dorsal Premotor Cortex Ventral Premotor Cortex |
100X oil immersion | 1/80 | 5 × 5 | 1000 × 1000 | 5 | 1 |
| Bromodeoxyuridine (BrdU) | Subventricular Zone Subgranular Zone |
60X oil immersion | 1/20 | 150 × 90 | 150 × 90 (exhaustive) | 15 | 1 |