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. 2018 Mar 6;37(20):2714–2727. doi: 10.1038/s41388-018-0133-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 3 — EZH2 depletion promotes differentiation and induces NTRK1 in NB cells. shEZH2-1 was introduced into NGP, SK-N-DZ, and TGW NB cells by a lentivirus system and total RNA collection/assessment of neurite extension were performed 5 (SK-N-DZ and TGW) or 8 (NGP) days after infection. The results are representative of at least three independent experiments. a NB cell differentiation-related gene expression was assessed by RT-PCR. b qPCR analysis of NTRK1. c, d Neurite extension was assessed by microscopy. The percentages of neurite-extending cells were counted. Data are presented as the mean ± s.d. from at least three independent experiments