Fig. 5.

EZH2 KD-induced neurite extension is canceled by the depletion of NTRK1. a, b NB-39-nu cells were infected with a mock- or shEZH2-1-expressing lentivirus 2 and 3 days after NTRK1 siRNA transfection. EZH2 and NTRK1 expression was assessed by semi-quantitative RT-PCR (a) and qPCR (b). c Images of EZH2 and/or NTRK1 knockdown NB-39-nu cells. The percentages of neurite-extending cells were counted. Data are presented as the mean ± s.d. from at least three independent experiments. d Photos of and percentages of neurite-extending NB-39-nu cells treated with shEZH2 lentivirus and/or NGF. Cells were infected with shEZH2 lentivirus, cultured for 2 days, and cultured for 3 more days after the addition of NGFbeta (100 ng/ml). Neurite extension was assessed as described in Methods, and NTRK1 expression was analyzed by semi-quantitative RT-PCR