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. 2018 May 16;8:7614. doi: 10.1038/s41598-018-25910-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GmBEHL1 encodes a functional ortholog of Arabidopsis BEH1. (a and b) GmBEHL1 interacts directly with GmBIN2. (a) Results of a Y2H assay to detect GmBEHL1 and GmBIN2L interactions. Yeast cells co-transformed with pGADT7/pGBKT7-GmBEHL1, pGADT7-GmBIN2/pGBKT7-GmBEHL1, and pGADT7-GmBIN2/pGBKT7 were grown on selective media lacking Leu and Trp (SD/−2) to check for transformation. The cells were subsequently grown on selective media lacking Ade, His, Leu, and Trp (SD/−4) to detect protein-protein interactions. (b) Results of a BiFC assay to detect the interaction of GmBEHL1 with GmBIN2. GmBIN2 and GmBEHL1 were fused to the N-terminus of YFP (nYFP) and C-terminus of YFP (cYFP), respectively. Bars = 25 μm. (c) qRT-PCR analysis of putative BR biosynthetic genes (GmCPDs and GmDWF4s) in roots transformed with EV1 and 35S:GmBEHL1 at 2 DAI (n = 6). The transcript amounts in each sample were normalized to those of ELF1b. The expression levels shown are the means ± SDs from three replicates. Asterisks represent statistically significant differences compared to empty vector (Student’s t-test; *p < 0.05, **p < 0.01, and ***p < 0.001).