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. 2018 May 16;8:7647. doi: 10.1038/s41598-018-26052-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Experimental study design. JDP2-overexpressing mice were fed for four weeks with doxycycline (Dox). Then Dox-feeding was stopped and JDP2 was overexpressed for one or five weeks. Parameters related to cardiac performance were analysed. (B) Evaluation of JDP2 overexpression. mRNA expressions of JDP2 were evaluated. Data are means ± SD. *Differences from WT-animals with p ≤ 0.05, n = 8. Furthermore, JDP2 protein from 5 weeks overexpressing hearts was detected in western blots by HA antibodies. Vinculin was used as loading control.